Exposure of <i>Agrobacterium tumefaciens</i> to agroinfiltration medium demonstrates cellular remodelling and may promote enhanced adaptability for molecular pharming

Agroinfiltration is used to treat plants with modified strains of Agrobacterium tumefaciens for the purpose transient in planta expression genes transferred from bacterium. These encode valuable recombinant proteins therapeutic or industrial applications. Treatment large quantities industrial-scale protein production exposes bacteria (harboring interest) agroinfiltration medium that devoid nutrients and carbon sources prolonged periods time (possibly upwards 24 h). Such conditions may negatively influence bacterial viability, infectivity plant cells, target production. Here, we explored role timing culture preparation using mass spectrometry-based proteomics define changes cellular processes. We observed distinct profiles associated treatment exposure timing, including significant involved pathogenesis, motility, nutrient acquisition systems as adapt new environment. data suggest a progression towards increased remodelling over time. In addition, described growth- environment-specific processes time, underscoring interconnectivity pathogenesis chemotaxis-associated transport metabolism. Overall, our results have important implications transiently expressed products, suggests enhanced infection cells.